<i>Sporosarcina pasteurii</i> can Efficiently Precipitate Calcium Carbonate at High Salt Concentration

The present work explores the adaptive behavior of well-known ureolytic bacterial strain namely Sporosarcina pasteurii for microbial-induced calcite precipitation (MICP) under saline environment. MICP activity was observed up to 10% NaCl supplementation and confirmed by several characterization techniques viz. scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA). urease did not show appreciable decline till 5% but beyond that drastic reduction though process continued. These results indicated a shift in biological pathway at higher salt concentrations. This estimation ammonium ion concentration which approximately 4 µg/ml with as compared 8.5 0% supplementation. To provide an insight on nature protein expressed, combination liquid chromatography-mass spectrometry (nano-LC-MS) approaches along exponentially modified abundance index (emPAI) methodology were explored. findings suggest presence significant number osmoregulatory proteins substantiating halophilic adaptation S. bicarbonate transport mediated levels salinity. Further, co-precipitation CaCl2 MgSO4 also SEM energy dispersive spectroscopy (EDS) mapping environment thus efficacy strain.